SERUM PROHEPCIDIN AND INTERLEUKIN-6 IN CHLORAMPHENICOL CHALLENGED LIVER OF WISTAR RAT MODELS

The role of the disturbances of hepridin metabolism, the master regulator of iron homeostasis synthesized in the liver, has been raised in cases of impaired liver function. The aims of this study were to evaluate the serum levels of prohepcidin, serum iron, interleukin-6, liver enzymes: aspartate transaminase (AST), alanine transaminase [ALT] and alkaline phosphatase (ALP) and total bilirubin, on chloramphe-adminisstered Wistar rat models, and also investigate the ameliorative property, if any, of Telfairia occidentals on chloramphenlcol-induced hepatocyte damage. The hepatocytes of 120 male Wistar rats (130-150g) were challenged in clinical conditions of chloramphenicol administration. Prohepcidin and interleukin-6 were measured by an enzyme linked Immunosorbent assay (ELISA) technique, and AST, ALT, ALP and total bilirubin were measured spectrophotometrically. 60 rats served as controls, while 60 rats were test animals. The control sub groups were given Telfairia occidentals leaf attract (50 mg/ml) and ferric hydroxide (50 mg/5ml) respectively with rat chow, for 21 days, while the test groups were administered Chloramphenicol (66mg/kg body weight) for 21 days, with normal feeding and treated as control sub-groups,A significant decrease (p 0.05) in serum prohepcidin levels and IL-B, was observed in chloramphenicol administered rat models, while 6erum AST, ALT, and ALP activities were elevated (p 0.05) in the rat models. Administration of Iblfairia ocddeniahs to the rat models greatly improved liver function and enhanced humoral immunity of the test animals. In conclusion, serum prohepcidin level was decreased in chloramphenicol-induced liver impairment and Telfairia occidentalis had restorative and Immune boosting properties.