Effect of China berry crude extracts on broad mites in vivo and in vitro

Tissue cultures were established for leaf and stem of china berry (Melia azedarach) tree. Using MS media the best regulator to form callus were 6mg/l BAP, all other concentrations did not give callus. The crude extracts from leaves and callus established from leaves were extracted with water and ethanol with different concentrations. In ethanol extracts the least concentration 0.0001 half of the treated parasites were killed in 24 hours while the number increases as the concentration increase. However, in callus the ethanol extracts were much higher about 8.5 were killed in the above concentration. In water extracts the least concentration 0.0001 killed half of the treated parasites in 24 hours. This number was increased 8 or 9 in 48 and 72 hours respectively. These results give us preliminary idea about the biological control of this dangerous parasite