Study the toxic effect of non-bound colicins extracted from Escherichia coil on transplanted Murine adenocarcinoma (AM3)

Four types of non-bound colicins (H5, H9, H13, H19) extracted from intestinal normal flora E.coli isolated from apparently healthy people’s stool. Then cytotoxic effect of colicins on transplanted murine adenocarcinoma (AM3) was studied. The results showed,that the four colicins had inhibitory effects on growth of AM3 cells when injected intratumorally at (200) mg/kg. Percentages of tumor inhibition were (98.79, 90.45, 90.08 and 88.26) % for colicins (H19, H13, H9 and H5) respectively. Intraperitoneal injection caused less inhibition, (57.47, 36.25, 60.26 and 53.25) % respectively using a daily dose of (200) mg/kg for period of 24 days.