Title of article :
Spatial and temporal changes in the PGE2 EP2 receptor in mice hippocampi during postnatal development and its relationship with cyclooxygenase-2
Author/Authors :
Jung ، Hyo Young Department of Veterinary Medicine - Institute of Veterinary Science - Chungnam National University , Kim ، Woosuk Department of Anatomy - College of Veterinary Medicine, Veterinary Science Research Institute - Konkuk University , Hahn ، Kyu Ri Department of Anatomy and Cell Biology - College of Veterinary Medicine, Research Institute for Veterinary Science - Seoul National University , Nam ، Sung Min Department of Anatomy - School of Medicine - Wonkwang University , Yi ، Sun Shin Department of Biomedical Laboratory Science - College of Medical Sciences - Soonchunhyang University , Kwon ، Hyun Jung Department of Biochemistry and Molecular Biology - Research Institute of Oral Sciences, College of Dentistry - Gangneung-Wonju National University , Kang ، Min Soo Department of Anatomy - College of Veterinary Medicine, Institute of Veterinary Science - Kangwon National University , Choi ، Jung Hoon Department of Anatomy - College of Veterinary Medicine, Institute of Veterinary Science - Kangwon National University , Kim ، Dae Won Department of Biochemistry and Molecular Biology - Research Institute of Oral Sciences, College of Dentistry - Gangneung-Wonju National University , Yoon ، Yeo Sung Department of Anatomy and Cell Biology - College of Veterinary Medicine, Research Institute for Veterinary Science - Seoul National University , Hwang ، In Koo Department of Anatomy and Cell Biology - College of Veterinary Medicine, Research Institute for Veterinary Science - Seoul National University
Abstract :
Objective(s): Prostaglandin E2 E-prostanoid 2 receptor (PGE2 EP2), downstream of cyclooxygenase-2 (COX-2), plays an important role in inflammatory responses, but there are some reports about synaptic functions of COX-2 and PGE2 EP2 in the hippocampus. Materials and Methods: C57BL/6J mice were sacrificed at postnatal days (P) 1, 7, 14, 28, and 56 for immunohistochemical staining for EP2 and doublecortin as well as western blot for EP2. In addition, COX-2 knockout and its wild-type mice were euthanized for immunohistochemical staining for EP2. Results: EP2 immunoreactivity was observed in the majority of the cells in the dentate gyrus at P1 and P7, while at P14, it was detected in the outer granule cell layer and was confined to its subgranular zone at P28 and P56. EP2 protein levels in the hippocampal homogenates were also highest at P7 and lowest at P56. EP2 immunoreactivity was partially colocalized, with doublecortin (DCX)-immunoreactive neuroblasts appearing in the mid-zone of the granule cell layer at P14 and in the subgranular zone of the dentate gyrus at P28. Co-localization of EP2 and DCX was significantly decreased in the dentate gyrus in the P28 group compared with that in the P14 group. In COX-2 knockout mice, EP2 immunoreactivity was significantly decreased in the hippocampal CA1 region (P=0.000165) and dentate gyrus (P=0.00898). Conclusion: EP2 decreases with age, which is expressed in DCX-immunoreactive neuroblasts in the dentate gyrus. This suggests that EP2 is closely linked to structural lamination and adult neurogenesis in the dentate gyrus.
Keywords :
Dentate gyrus , Growth and Development , Hippocampus , Mice , Prostaglandin E EP2 subtype
Journal title :
Iranian Journal of Basic Medical Sciences
Journal title :
Iranian Journal of Basic Medical Sciences
