Role of Pili and Polysaccharide in adherence of Pseudomonas aeruginosa to mammalian epithelial cells

Adherence capability of Pseudomonas aeruginosa isolated from Iraqi patients was tested in accordance to their capability to adhere to epithelial cells. A range of adherence ability was found: The highest capacity with mean number of adhering bacteria of (13.5) was noticed. A sparse content or alginate was detected in these isolates analysis of pilin protein isolated from cultures exposed to ciprofloxacin antibiotic at sub MIC, level using polyacrylamide gel electrophoresis indicated that treatment of cells with 3/4 MIC show highest activity as compared with others. Results of protein profile followed the same trend of that in adhesion experiment which allows hypothesizing that these isolates are piliated, not mucous.