PCR Detection of Aspergillus flavus Isolates for Aflatoxin B1 producer

The ability of five Aspergillus flavus that produce Aflatoxin B1 have been detected using coconut medium as substrate. Chromatographical analysis by TLC and HPLC revealed that, three out of five isolates were a good producer for the Aflatoxin B1. In this study, rapid assessment of five isolates of A. flavus was accomplished using an indigenously designed primer pair for the Aflatoxin regulatory gene aflR in polymerase chain reaction (PCR). Specificity was assayed in pure culture systems using DNA extracted from five different A. flavus isolates as PCR template. Positive amplification was achieved only with DNA from A. flavus that produce Aflatoxin B1.