اكثار ابصال النرجس Narcissus tazetta L خارج الجسم الحي

In vitrotechnique was used to propagate Narcissus tazettaL. Two approaches were used. The first explantsof basal discs and scale leaves from bulbs were cultured in MS medium containing different concentrations of BA and NAA. Cultures were incubated in light and dark.Results showed that asignificant increasing in shoot numbers and lengths in both types lfexplants. Treatments including; light + 7.0 mg / L BA + 0.0 mg / L NAA (15.8 shoots) and light + 3.0 mg / L BA + 1.0 mg/L NAA (9.04 cm) weresignificant for the basal disc. while the treatments: light + 3.0 mg / L BA + 0.0 mg /L NAA (12.5 shoots ) and light + 1.0 mg/ L BA + 0.0 mg / L NAA (5.02 cm) were the best for scale leaves. Callus was induced largely in explants cultured in dark and high concentrations of NAA. The second experiment: shoots from above were cultured in MS medium containing sucrose 3 or 6% with IBA 0.5,1.0 ,1.5mg / L for bulb letsformation. The highest numbers of bulb lets3.2, 3.4were observed in: 3% sucrose + 1.5 mg / L IBA and 6% sucrose + 1.0 mg / L IBA respective. On the other hand, the bulb letdiameter 7.42 mm and weight 320.4 mg were observed in: 3% sucrose + 1.5 mg/ L IBA and 3% sucrose + 0.5 mg / L IBA respectively. The formed bulb letswere transferred to peatmoss plus river soil media,which then successfully grown and gave vegetative shoots in the percent 98%. Abbreviations: BA(6-benzyladenine) ; NAA(α-naphthalene acetic acid );IBA ( Indole –butyric acid); MS( Murashige and Skoog medium).