اﻟﻔﻌﺎﻟﯿﺔ اﻟﺘﺜﺒﯿﻄﯿﺔ ﻟﻤﺴﺘﺨﻠﺼﺎت اﻟﻜﺎﻟﺲ واوراق ﻧﺒﺎت اﻟﻜﺎردﯾﻨﯿﺎ Gardenia jasminoides ﺑﺎﺳﺘﺨﺪام ﺑﻌﺾ اﻟﻤﺬﯾﺒﺎت اﻟﻌﻀﻮﯾﺔ ﻓﻲ ﻧﻤﻮ ﺑﻌﺾ اﻷﻧﻮاع اﻟﺒﻜﺘﯿﺮﯾﺔ اﻟﻤﺮﺿﯿﺔ واﻟﺨﻤﺎﺋر

The study was conducted to evaluate the inhibitory activity of methanol extract of Gardenia jasminoidesleavescompared with leaf crude extracts for some organic solvents namely Methanol, Ethanol, Petroleum ether, Asetone and Chloroform on growth of some pathogenic bacteria and yeast, which included four gram positive isolates Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes and Bacillus cereusand gram negative isolates Escherichia coli,Salmonella typhi, Proteus vulgaris andPseudomonas aeruginosa andsomeyeastsCandida albicansandSaccharomyces boulardii, by using well diffusion method.The inhibitory activity of extracts in the tested bacterial strains and yeasts was varied according to the type of extracting solvents and are tested microorganisms. Themethanol callus extract which grown on Murashige and Skoog(MS) media by using (Naphthalen acitic acid)NAA and (Benzyle adenine)BA as growth regulatorhighly effective as compared to the other extracts as for inhibition of three gram positive bacteria and three gram negative bacteria,which include Staphylococcus aureusand, Proteus vulgaris,followed by acetone and ethanolic extractswhich include two gram positive bacteria and two gram negative bacteria. All extractshad highly effect in growth of Candida albicanswhileall crude extractsdidn’t show any sensitivityagainstSaccharomyces boulardii, and when we’d done(High Performance Liquid Chromatography) HPLC test for detection of some active compound we found Quinic acid, Iridiods glycosides and Crocin which its rate in fresh callus was higher than fresh leaves