Title of article :
ﺗﺄﺛﯿﺮ ﻓﻌﺎﻟﯿﺔ اﻟﻤﺮﻛﺒﺎت اﻟﻤﻨﺘﺠﺔ من اﻟﻄﺤﻠﺐ Gloeocapsa punctata ﺗﺠﺎه ﺑﻌﺾ اﻟﺒﻜﺘﺮﯾﺎ واﻟﻜﺸﻒ عن ﺑﻌﺾ اﻷﺣﻤﺎض اﻟﺪھﻨﯿﺔ فيه
Author/Authors :
علي, غيداء حسين عبد جامعة المستنصرية - كلية العلوم, العراق , شوكت, دعاء سهيل جامعة المستنصرية - كلية العلوم, العراق , لفتة, علي رزاق الجامعة المستنصرية - كلية العلوم, العراق
Abstract :
n this study Gloeocapsa punctatawas isolated, purified and identified from the object of the home garden in Zayouna. BG-11 culture media was used for their cultivation in suitable laboratory conditions 25c°, 200μE/m2/sec for 16:8 hrs. Light: dark. Each culture was harvested at the end of exponential phase. Organic solvents used for extraction was Chloroform: methanol at 2:1 to extract,study aims to test the effectiveness of extracted interacellular (biomass) and overseas cellular Extracellular filtrate cellular and used solvent organic (Chlorferom: Methanol 95%) and by 1:2 and test their effectiveness against 10 strains of bacteria which: Bacillussubtilis , Staphylococcus aureus, Staphylococcus epidermidiss, Enterococcus faecium, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Shigella flexeneriand Serratiasp. Agar diffusion method was used. Results showed that the extracellular products which extracted was best than intercellular product.The gram positive bacteria studied revealed higher susceptibility to attack by the intracellular and extracellular extracts comparing with the gram negative bacteria. The extracellular extraction revealed higher antibacterial activity against Staphylococcus epidermidiss the of inhibition zone was 28 mm. But the intercellular extract revealed higher antibacterial activity against Enterococcus faecium and the average inhibition zone was 22 mm. However, results showed that gram negative bacteriaextracellular products extract has the antagonistic activity against Klebsilla pneumonia with25 mm inhibition zone.Also been detected on some fatty acids produced by the algae G. punctatawhich Palmitic, Stearic, Arachidic, Linoleic and Linolenic. The highest rate is fatty acid Linoleic 57% of the extract intercellular while the rate of 30% of the acid fatty Arachidic to intercellular abroad
Keywords :
Blue-greenish algae , Algae extraction , antibacterial , inhibito
Journal title :
Journal Of Biotechnology Research Center
Journal title :
Journal Of Biotechnology Research Center