Author/Authors :
Hashemi, Mohammad zahedan university of medical sciences - Department of Clinical Biochemistry, زاهدان, ايران , Mehrabifar, Hamid zahedan university of medical sciences - Bou Ali Hospital - Research Center for Infectious Diseases and Tropical Medicine, زاهدان, ايران , Homayooni, Fatemeh zahedan university of medical sciences - Bou Ali Hospital - Research Center for Infectious Diseases and Tropical Medicine, زاهدان, ايران , Naderi, Mohammad zahedan university of medical sciences - Bou Ali Hospital - Research Center for Infectious Diseases and Tropical Medicine, زاهدان, ايران , Montazerifar, Farzaneh zahedan university of medical sciences - School of Medicine - Department of Nutrition, زاهدان, ايران , Ghavami, Saeid University of Manitoba - Manitoba Institute of Cell Biology - Department of Biochemistry and Medical Genetics, Canada
Abstract :
It has been established that overproduction of reactive oxygen species (ROS) occurs during hemodialysis causing oxidation of proteins. Alpha-1-antitrypsin is the major circulating antiprotease which contains methionine in the active site. The aim of the present study was to measure the level of serum trypsin inhibitory capacity (sTIC) in hemodialysis patients. This case-control study was performed in 52 hemodialysis patients and 49 healthy controls. sTIC was measured by enzymatic assay. The sTIC was significantly (P 0.001) lower in hemodialysis patients (1.87 ± 0.67 µmol/min/mL) than healthy controls (2.83 ± 0.44 µmol/min/L). Reduction of sTIC may be due to the oxidation of methionine residue in the reactive site of alpha-1 antitrypsin.
Keywords :
Alpha , 1 Anitrypsin , Hemodialysis , Chronic renal failure , Serum trypsin inhibitory capacity
