• Title of article

    Pronase-free B-cell flow-cytometry crossmatch

  • Author/Authors

    Hajeer, AH King Saud bin Abdulaziz University for Health Sciences - College of Medicine,Laboratory Medicine - Department of Pathology, Saudi Arabia , Saleh, S King Abdulaziz Medical City - Laboratory Medicine - Department of Pathology, Saudi Arabia , Sutton, P King Abdulaziz Medical City - Laboratory Medicine - Department of Pathology, Saudi Arabia , Shubaili, A Laboratory Medicine - Department of Pathology, Saudi Arabia , Anazi, H Laboratory Medicine - Department of Pathology, Saudi Arabia

  • From page
    662
  • To page
    665
  • Abstract
    Detection of anti-class II antibodies by panel response assay (PRA) and flow cross­match techniques carries an important value in terms of graft function. Even low levels of pre­formed alloantibodies to HLA class II antigens represent a risk of rejection. We present here a method for blocking non-specific flow crossmatch reactions using pooled, heat-inactivated rabbit serum. This method shows very low background and minimal non-specific reactions. In addition, it avoids the use pronase enzyme that can non-specifically digest different cell surface proteins.
  • Journal title
    Saudi Journal of Kidney Diseases and Transplantation
  • Journal title
    Saudi Journal of Kidney Diseases and Transplantation
  • Record number

    2674772

    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=10&DC=2674772