Title of article :
اﻹﻛﺜﺎر اﻟﺨﻀﺮي اﻟﺪﻗﯿﻖ ﻻرﺑﻌﺔ اﺻﻨﺎف ﻣﻦ اﻟﺒﻄﺎطﺎ SolanumtuberosumL. ﺧﺎرج اﻟﺠﺴﻢ اﻟﺤﻲ Micro propagation of four Potato Cultivars Solanum tuberosum L. in vitro
Author/Authors :
الحسيني, زينب عبدالجبار حسين وزارة العلوم و التكنولوجيا - دائرة البحوث الزرائية, العراق , يوسف, شذي عايد وزارة العلوم و التكنولوجيا - دائرة البحوث الزراعية و تكنولوجيا الغذاء, العراق , عبداللطيف, شيماء وزراة العلوم و التكنولوجيا - دائرة البحوث الزراعية, العراق , عبد, نورا صاحب وزارة العلوم و التكنولوجيا - دائرة البحوث العراقية, العراق , عبدالجبار, تغريد وزارة العلوم و التكنولوجيا - دائرة البحوث الزراعية, العراق , بندر, هيفاء محسن وزارة العلوم و التكنولوجيا - دائرة البحوث الزراعية, العراق
Abstract :
Vegetative buds excised from tubers for four varieties of potatoes namely: Arnova, Brovento, Rivira and Bureen (after breaking the dormancy phase) which sterilized with 2% Sodium hypochlorite for 10 minutes and cultured in the MS mediumsupplemented with 0.5 mg/l of IAA or IBA with different concentration of BA, as well as control treatment in the initiation stage,while different concentrations of BA (0.0, 0.5, 1.0, 1.5, 2 mg/l) with 0.5 IAA mg/l in multiplication stage. All cultures incubated at a temperature of 2 ± 25º C and an illumination of 1000 lux for 16 hours/ day. Data ofmean number ofbranches shoot per plant and their length and leaves fortwo stages were investigated. The results showed that BA+ IAA at 0.5 mg/L for each one, gave the highest initiated percentage, shoot per plant ,length, reached 75%, 1.5 shoot / bud,2.21cm and 3.88,leaf/ bud, respectively. Results of multiplication indicated that there was a significant interaction effect of both cultivars and BA concentration on all characterizes, Based on this interaction, Arnova cultivars at 1.5 mg/L BA showed the highest mean of shoot per plant (4.0) While Rivera cultivars at 0.5 mg/L BA gave highest mean of length, leaves per plant reached 6.45cm,7.75 leaf/plant respectively.
Keywords :
Potato , imitation and multiplication , BA , IAA , IBA , in vitro
Journal title :
Journal Of Biotechnology Research Center
Journal title :
Journal Of Biotechnology Research Center