Title of article :
Isolation and Identification the Fungus Trichophyton violaceum from Human SkinSpecimens in Iraq and Study Efficiency Antibacterial and somePlant Essential Oils
Author/Authors :
al-jeboury, ghydaa h. al-nahrain university - biotechnology research center, Iraq , muhsin, athraa h. university of kufa - college of science, Iraq , risan, mohsen h. al-nahrain university - college of biotechnology, Iraq
Abstract :
This study was aimed to isolate and identify the fungus Trichophyton violaceum from human skinspecimens and evaluate the activity of antibacterial and plant essential oils. The results showed of directmicroscopy by using KOH examination and morphological identification, nine samples from skin werepositive in KOH test. Microscopical examination appeared that colonies of T. violaceum were coarse withabundant aerial mycelium on SDA, growth rates 6-8cm/14 days. The color changes with age from white inthe middle to brownish yellow at the edges which was more intense on the reverse side. Susceptibility test to antibacterial showed that 100% of T.Violaceum T2 isolate was resistant to TE (Tetracycline), AK (Amikacin), CTX (Cefotaxime), CFM (Cefixime), CIP (Ciprofloxacin), ERY (Erythromycin) and STR (Streptomycin).Also results showed that all plant essential oils at different concentrations significantly inhibit growth of T. violaceum T2. However, the peppermint oil, Myrrh oil, Cardamom oil, Chamomile oil and Castor oil at highest concentration (25mg / ml) caused highest reduction of mycelia growth (100%) followed by Olive oil (67.3%) and Clove oil (64%) at the same concentration, compared with the control treatment.
Keywords :
Trichophyton violaceum , skin , antibacterial , essential oils.
Journal title :
Journal Of Biotechnology Research Center
Journal title :
Journal Of Biotechnology Research Center