Author/Authors :
Jawdat, Dunia King Saud bin Abdulaziz University for Health Sciences - King Abdullah International Medical Research Centre, Saudi Arabia , Al Saleh, Salwa King Saud bin Abdulaziz University for Health Sciences - King Abdulaziz Medical City - Department of Pathology, Laboratory Medicine, Saudi Arabia , Sutton, Paul King Saud bin Abdulaziz University for Health Sciences - King Abdulaziz Medical City - Department of Pathology, Laboratory Medicine, Saudi Arabia , Al Anazi, Hanan King Saud bin Abdulaziz University for Health Sciences - King Abdulaziz Medical City - Department of Pathology, Laboratory Medicine, Saudi Arabia , Shubaili, Abdullah King Saud bin Abdulaziz University for Health Sciences - King Abdulaziz Medical City - Department of Pathology, Laboratory Medicine, Saudi Arabia , Uyar, F. Aytul Istanbul University - Istanbul Medical Faculty - Department of Physiology, Turkey , Hajeer, Ali H. King Saud bin Abdulaziz University for Health Sciences - College of Medicine, King Abdulaziz Medical City - Department of Pathology, Laboratory Medicine, Saudi Arabia
Abstract :
The typing for HLA-C in transplantation was rather neglected in the past. However,several recent studies have emphasized its role in transplantation and its association with the outcome. Serological typing of HLA-C could identify only a limited number of HLA-C antigens,resulting in a number of HLA-C blanks. This was mainly due to the low expression of surface HLA-C and the small number of available specific anti-sera. Performing molecular methods has identified new HLA-C alleles and filled the blank of most serological typed antigens. In this study,we compared serological and molecular typing of HLA-C in two cohorts of healthy Saudis. Our serological typing method identified HLA-C1-7 with different frequencies,23.5% of the alleles were not identified and thus defined as blank. Using the SSP molecular method,all samples were typed and all alleles were defined. Both methods showed that C*07 and C*06 have the highest frequency in the Saudi population. Our study emphasizes the importance of molecular methods in identifying all possible HLA-C alleles.
