Author/Authors :
farboodniay jahromi, mohammad ali shiraz university of medical sciences - medicinal plants processing research center, Shiraz, Iran , emami, amir shiraz university of medical sciences - burn wound healing research center, amir-al-momenin burn hospital , school of medicine - department of bacteriology virology, department of microbiology, Shiraz, Iran , nazeri, raha shiraz university of medical sciences - school of pharmacy - department of pharmacognosy, Shiraz, Iran , pirbonyeh, neda shiraz university of medical sciences - burn wound healing research center , amir-al-momenin burn hospital, school of medicine - department of bacteriology virology, department of microbiology, Shiraz, Iran
Abstract :
The present study deals with antibacterial and antioxidant screening of ethanolic leaf extract and various fractions of Lonicera nummularifolia, an Iranian endemic plant of Caprifoliacea family. The ethanolic extract was prepared using soxhlet extraction procedure and fractionation of the extract was performed by a continuous liquid-liquid extraction method. Total phenol, flavonoid and triterpenoid content of the extract were determined, using standard spectrophotometric methods. DPPH and FRAP assays were conducted to evaluate the antioxidant capacity. Antibacterial activity of total ehanolic extract and fractions were evaluated by measuring MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) against four standard bacterial strains, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosae and Acenitobacter baumannii, using broth microdilution method. Total phenol content of hydroalcoholic extract was found to be higher than flavonoid. Results of antioxidant assays indicated that ferric reducing antioxidant power of the extract was promising. Results of antibacterial screening declared the lowest MIC value for ethanol extract and therefore higher bacteriostatic activity against the tested bacterial strains compare to chloroform and ethyl acetate fractions. Ethanolic extract also revealed significant bactericidal activity among all the tested extracts. Overall inspection of the results declared moderate DPPH free radical scavenging while promising ferric reducing activity and antibacterial properties of L. nummularifolia which is worthy of further detailed investigations. Isolation and characterisation of active substances of the extract are interesting approaches in order to validate antibacterial and ferric reducing properties of the plant.
