Attenuation of incident light sources (two flow tubes in one geometrical flow cell assembly: First with eleven sources while the second is covered by six sources) in CFIA for the determination of Copper (II) ion

A highly accurate, simple, sensitive and fast developed method was used for the determination of copper (II) ion using a homemade NAG-ADF-300-2 system with continuous flow injection analysis system. This method is based on reaction between copper (II) ion and rubeanic acid to obtain a black precipitate for the ion-pair complex. Turbidity was measured via the attenuated of incident light by precipitated at 0-180°. Optimum parameters were studied to increase the sensitivity for newly developed methodology of copper (II) ion -rubeanic acid system. The linear range for calibration graph was 1-25 mmol.L^-1 for cell A and 1-30 mmol.L^-1 for cell B, and LOQ 27.0538 μg /78.5 μL for cell A and 18.5539 μg /78.5 μL for cell B respectively with correlation coefficient (r) 0.9979 for cell A and 0.9992 for cell B, RSD % was lower than 0.5%, (n=8) for the determination of copper (II) ion at concentration 18, 23 mmol.L^-1 for cell A and 12, 19 mmol.L^-1 for cell B, respectively. A comparison was made between the newly developed method with UV-Spectrophotometric method as a classical method by tow type of statistical analysis for comparison the means and variance from different methods. The comparison of data revealed that long distance chasing photometer (NAG-ADF-300-2) was the choice with excellent extended detection, wide application and more sensitive.