Author/Authors :
Hanachi, Nadjet Laboratory of Applied Biochemistry - Department of Biology , Charef, Noureddine Laboratory of Applied Biochemistry - Department of Biology , Baghiani, Abderrahmane Laboratory of Applied Biochemistry - Department of Biology , Khennouf, Seddik Department of Biology - Laboratory of Phytotherapy Applied to Chronic Diseases , Derradji, Yacine Laboratory of Applied Biochemistry - Department of Biology , Boumerfeg, Sabah University Ferhat Abbas - Faculty of Sciences - Department of Biology , Harzallah, Daoud Laboratory of Applied Biochemistry - Department of Biology , Arrar, Lekhmici University Ferhat Abbas - Faculty of Sciences - Laboratory of Applied Biochemistry, Algeria
Abstract :
Objectives: To search whether xanthine oxido-reductase (XOR) present in the synovium is also liberated, to determine its activity in synovial fluid and to establish a possible relationship between XOR levels in rheumatoid arthritis (RA) and non-RA patients.Methods: This study was carried out in the Laboratory of Immunology, University Ferhat Abbas, Setif, Algeria from 2001-2008. This study is a retrospective controlled study matching cases with RA to non rheumatoid joint inflammations. Synovial fl uid (SF) samples were collected with consent of the patients, at Setif University Hospital, from adults suffering from RA (n=36) or only with joint inflammations (n=52). /iter its detection in SF with indirect enzyme-linked immunosorbent assay (ELISA) and dot-immunobinding, using anti-bovine XOR as first antibodies, XOR was assayed with capture ELISA.Results: Xanthine oxidoreductase is found in all studied SF. Capture ELISA showed levels up to 0.762 and 0.143 mg/mL in SF of RA and other joint inflammations patients, respectively. In most cases, more than 50% of synovial XOR is present as oxidase form. Positive correlation was observed between enzyme level and the disease severity since RA patients had a significantly high enzyme amount compared to patients with other less severe arthritic pathologies.Conclusion: These results suggest that the enzyme could well be involved in joint inflammation probably by producing reactive oxygen species.
