Development of a new high performance liquid chromatography method for measurement of coenzyme Q10 in healthy blood plasma

Objectives: To improve a new high performance liquid chromatography (HPLC) method for coenzyme Q10 (CoQ10) measurement in human plasma. Methods: We conducted this study in the Chemistry and Biochemistry Department, College of Medicine, Al-Nahrain University, Baghdad, Iraq from September 2004 to July 2005. Sixty healthy volunteers aged 28.51±7.30 years were participated in this study. All participants were non-smokers and under no any treatment for the last 3 days and fast for 12 hours before analysis. Coenzyme Q10 levels were measured by improved isocratic reversed phase HPLC technique after one-step ice-cold 1-propanol extraction step. Results: The ultra violet (UV) detection, developed HPLC method gives a good linearity range between 0.1-4.0 mg/L. The coefficients of variations (CVs%) of within-day precision for CoQ10 in human plasma were 0.2-3.90%. Analytical recoveries were 95.5-101.30%, whereas the average CV% of CoQ10 reproducibility was 0.959%. Conclusion: A new improved rapid, precise, and sensitive HPLC method for the quantitation of CoQ10 is suitable for analysis of blood plasma samples.