Invitro Study of the Antimicrobial Effectives of Commercially Available Toothpastes

Dental caries are formed by the increase in number in dental plate of such bacteriae Streptococcus mutans, Lactobacillus spp. The bacteriae produce acid by breaking down the carbohydrates very quickly. Therefore, one of the objectives of the caries preventive interventions is to inhibit the formation of the acid. Candida albicans causes aphthous lesions in the mouth and also can cause frequent infections in those who having dental prostheses. The main cause of periodontal dental caries is that the bacteriae and dental plaque and the solution is oral hygiene including teeth brushing. For this reason this study analyzes the effects of toothpastes in different brands and different contents on these bacteriae. Baskent University students randomly selected from among 169 people applied to the survey to determine the toothpates to be tested. According to the survey, invitro comparative antimicrobial effectiveness of thirteen selected commercially available toothpaste on Streptococcus mutans, Lactobacillus acidophilus, Candida albicans were investigated by using the agar well diffusion method. Thirteen toothpastes are numbered regardless of the number of toothpastes preferred. Chlorhexidine mouthwash as positive control, blank wells as negative control were used. Bacterial dilutions were prepared as recommended as antibiogram preparation and these mixtures inoculated into Mueller Hinton feed lots with and without blood via swap. Wells of a microtiter plate is opened and tested toothpaste is put (6 wells for the each). Toothpastes placed into the wells were weighed and inhibition zone diameters were measured waiting after 24 hours at 37° C. The data obtained were evaluated statistically. According to the zone diameters, compared to controls, the number 2 toothpaste to L.acidophilus and C.albicans and number 8 toothpaste to S.mutans has a lesser effect. As percent activity is ev aluated (mm/g) none was effectiveas controls. The efficacy of toothpastes found to be lower than the positive control group. Accordingly the antimicrobial efficacy of toothpastes is not crucial. Mechanical brushing of the teeth is more critical to prevent tooth decay.