Author/Authors :
Karamehic, Jasenko University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Delic-Sarac, Marina University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Subasic, Djemo University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Jukic, Tomica Osijek University - Medical Faculty - Scientific Department of Biomedicine and Health, Croatia , Coric, Jozo University of Sarajevo - Clinical Biochemistry Clinical Center, Bosnia and Herzegovina , Panjeta, Mirsad University of Sarajevo - Clinical Biochemistry Clinical Center, Bosnia and Herzegovina , Drace, Zahida University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Zecevic, Lamija University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Mutevelic, Selma University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Dzananovic, Nejra University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Grcic, Nerima University of Sarajevo - Clinical Immunology Clinical Center, Bosnia and Herzegovina , Kesmer, Amila General Hospital “Prim.dr. Abdulah Nakaš”, Bosnia and Herzegovina
Abstract :
Introduction: In this study authors have analyzed the correlation between theIgG immunoglobulins in cerebrospinal fluid and the findings of oligoclonal bandson gel. Immunoglobulin IgG in cerebrospinal fluid (CSF) can be detected inneurological diseasses (infections and inflammatory neurological diseases and indemyelinating diseases, like multiple sclerosis (MS)). Quantitative IgG in CSF canbe expressed by different formulae Reiber (Reiber and Felgenhauer 1987), Tourtellotte(Tourtellotte 1970), Schuller (Schuller and Sagar 1983) and IgG Index (Linkand Tibbling 1977). In this study we used Reibergram. Qualitative CSF IgG can bemeasured by electrophoresis and isoelectric focusing (IEF). We used IEF for analysigCSF and seum because of its higher sensitivity. Aims of the study: To determine thecorrelation of immunoglobulins IgG positivity in CSF with the finding of oligoclonalbands on the gel. Material and methods:: The retrospective study based on dataprocessed in OJ Clinical Immunology KCUS. Patients were suspicious of multiplesclerosis according to clinical findings and magnetic resonance imaging. All CSFand serum samples were processed by nephelometry, isoelectric focusing on thegel. Statistical analysis of intrathecal synthesis was also performed according toReibergram. Results: Analyses were performed on 76 samples of cerebrospinal fluidand serum of patients from neurological clinic, suspected of multiple sclerosis. Wereceived following results: 42 samples tested had type 1. 25 samples tested showedtype 2. 3 samples had type 3. 5 samples had type 4. 1 sample had a fifth type. Whenwe compare these results with values obtained by intrathecal synthesis of which isdetermined by Reibergram we obtained the following values: 16 samples had intrathecalsynthesis of 20%–60%, 9 samples had a negative value of intrathecal synthesisof 10% or less. Discussion and Conclusion: For most patients with established MSwe found intrathecal humoral response, type two, and the number and arrangementof IgG bands generally does not change during the disease, because they reflectlong-term non-specific immune stimulation rather than a specific immune responsethat during infectious disease changes (quantitatively and qualitatively).
