Author/Authors :
Ata, Sadia Institute of Chemistry - University of the Punjab, Lahore 54590, Pakistan , Wattoo, Feroza Hamid University Institute of Biochemistry & Biotechnology - Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan , hmed, Mahmood A Institute of Chemistry - University of the Punjab, Lahore 54590, Pakistan , Wattoo, Muhammad Hamid Sarwar University Institute of Biochemistry & Biotechnology - Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan , Tirmizi, Ahmed Department of Chemistry - Quaid-i-Azam University, Islamabad 45320, Pakistan , Wadood, Abdul Department of Chemistry - The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
Abstract :
A sensitive, reliable and relative fast method has been developed for the determination of total zinc in insulin by atomic absorption spectrophotometer. This designed study was used to optimize the procedures for the existing methods. Spectrograms of both standard and sample solutions of zinc were recorded by measuring the absorbance at 213.9 nm for determination of total zinc. System suitability parameters were evaluated and were found to be within the limits. Linearity was evaluated through graphical representation of concentration versus absorbance. Repeatability(intra-day) and intermediate precision (inter-day) were assessed by analyzing working standardsolutions. Accuracy and robustness were experimented from the standard procedures. The percentage recovery of zinc was found to be 99.8%, relative standard deviation RSD 1.13%, linearity of determination LOD 0.0032µg/mL, and limit of quantization LOQ 0.0120 µg/mL. This developed and proposed method was then validated in terms of accuracy, precision, linearity and robustness which can be successfully used for the quantization of zinc in insulin.
Keywords :
Flame atomic absorption spectrophotometer , Zinc , Insulin , Method development , Method validation
