Identification of differences in virulence factors production from UV irradiated isolate of clinical Vibrio cholerae S

Mutants from the clinical isolate of Vibrio cholerae S were isolated after UV irradiation on the basis of antibiotic resistance for Rifampcin, Streptomycin and Klindamycin. Results revealed that the highest antibiotic resistant incidence is for Rifampicin after UV exposure for 15 minute. One thousand mutant isolates were examined and showed smaller sized colonies on TSA appeared as smooth and translucence with yellow color on TCBS agar and pale on MacConkey agar. The mutant isolates gave positive results with oxidase and string test. Autoagglutination range from high level order designated +++ to mild ++ and low level designated as +. Only 15% of Rifampicin resistant mutant isolates gave no agglutination phenomenon. UV treated isolates produced delayed type of proteases with no changes in haemolycin and lipases production while, slightly autoagglutination has been detected from these mutant. Twenty out of screened isolates produced 10-30U/ml of CT at non- permissive conditions. The toxin extract from mutant isolates symptoms such as muscle spasm, muscle cramp, exhaustion, trummer and tachycardia in mice after intraperitoneal injection. Erythemal effect and induration in the injected site of Guinea pig skin after intradermal injection remained even after more than one week, with pronounced bleeding detected in the injected area.