Abstract :
Fourty patients were included in this study all diagnosed clinically cholecystitis and cholecystitis with stone . Bile fluid and pieces of gallbladder were collected from the operating theater then cultured , growth of pathogenic bacteria were identified by standard bacteriologicale testes . Escherichia coil represent the most common pathogene (75%) in cholecystitis,(79%) in cholecystitis with stone while β-heaolytic streptococci(12.5%) in cholecystitis, (8.3%) in cholecystitis with stone , Klebsiella spp. (6.25%) in cholecystitis ,(12.5%) in cholecystitis with stone ,Proteus spp. were isolats from cholecystits pationts(6.25%).