امكانية استخدام مستضد Virulence Antigen كلقـــاح ضد البكتيريــــا Salmonella typhi فى حيوان المختبر

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The present study was conducted to investigate the presence of S. typhi in clinical samples such as blood &stool. The isolates was diagnosed depending on the biochemical tests and growth characteristics on selective and differential culture media. The chemical reaction and bioassay were positive. Also the vaccine sterility and safety were tested. The activity of Vi-antigen to stimulate the immune response in immunized mouse through a number of immune tests which are: phagocytosis, seroconversion and delayed hypersensitivity reaction, The result revealed that 107 is the best immunizing dose. After separation and purification of the vaccine prepared from S. typhi, LD50 was calculated by injected mice with the following concentrations (2.6× 104; 3.3× 107; 2.12× 107) in 0.1 ml respectively, and the 106, 107,108 were chosen to evaluate the best concentration the in stimulate immune response. Immune response in mice was evaluated using the following assays: Ab. titration assay, phagocytosis and delayed hypersensitivity. The results of this study confirmed that, Vi–vaccine in boosting dose after 8 days from vaccination is the best efficient and gave highest mean in phagocytosis, Ab. titration assay and significant increase in delayed hypersensitivity