Author/Authors :
Aljuffali, Ibrahim A. King Saud University - College of Pharmacy - Department of Pharmaceutics, Nanomedicine Research Unit, Saudi Arabia , Abul Kalam, Mohd. King Saud University - College of Pharmacy - Department of Pharmaceutics, Nanomedicine Research Unit, Saudi Arabia , Sultana, Yasmin Hamdard University - Faculty of Pharmacy - Department of Pharmaceutics, India , Imran, Ahamad King Saud University - King Abdullah Institute for Nanotechnology, Saudi Arabia , Alshamsan, Aws King Saud University - College of Pharmacy - Department of Pharmaceutics, Nanomedicine Research Unit, Saudi Arabia , Alshamsan, Aws King Saud University - King Abdullah Institute for Nanotechnology, Saudi Arabia , Alshamsan, Aws King Saud University - Prince Salman Bin Abdulaziz Chair for Kidney Disease, Saudi Arabia
Abstract :
Quantitative determination of gatifloxacin in tablets, solid lipid nanoparticles (SLNs) and eye-drops using a very simple and rapid chromatographic technique was validated and developed. Formulations were analyzed using a reverse phase SUPELCO 516 C-18-DB, 50306-U, HPLC column (250 mm · 4.6 mm, 5 lm) and a mobile phase consisting of disodium hydrogen phosphate buffer:acetonitrile (75:25, v/v) and with orthophosphoric acid pH was adjusted to 3.3 The flow rate was 1.0 mL/min and analyte concentrations were measured using a UV-detector at 293 nm. The analyses were performed at room temperature (25± 2 C). Gatifloxacin was separated in all the formulations within 2.767 min. There were linear calibration curves over a concentration range of 4.0–40 lg.mL 1 and correlation coefficients of 0.9998 with an average recovery above 99.91%. Detection of analyte from different dosage forms at the same Rt indicates the specificity and stability of the developed method.
