Staphylococcus aureus Klinik İzolatlarında Panton-Valentin Lökosidin Varlığının Araştırılması

Objective: In this study, the presence of Panton-Valentine Leukocidin (PVL) was investigated in the Staphylococcus aureus clinical isolates by PCR. Material and Methods: In this study, 130 MRSA and 20 methicillin-sus- ceptible S. aureus (MSSA) isolates were tested among various clinical samples between February 2006 and June 2008. Confi rmation of S. au- reus isolates and the methicillin resistance of these isolates were made by multiplex PCR. The presence of PVL was tested by both multiplex PCR and single target PCR. Results: The presence of PVL was determined in 3 isolates. Two of the isolates were from MRSA isolates, one was from MSSA isolates. One of the PVL positive MRSA isolate was recovered from a wound specimen, the other was from blood culture. PVL positive MSSA isolate was recovered from an urine specimen. All of the three PVL positive isolates were resist- ant to tetracycline. One of the PVL positive MRSA isolates which was re- covered from blood culture was resistant to gentamicin and ciprofl oxacin, while the other PVL positive isolate was susceptible. Conclusion: Further studies are needed to determine the prevalence and genetic characteristics of PVL positive isolates and to prevent dissemina- tion of PVL positive isolates both in the hospital environment and the community.