Title of article :
Using fish brain biomarkers to assess the neurotoxicity of aspartame artificial sweetener
Author/Authors :
Shirmardani, Aisan Department of Biology - Central Tehran Branch - Islamic Azad University, Tehran, Iran , Rafiee, Aras Department of Biology - Central Tehran Branch - Islamic Azad University, Tehran, Iran , Moghadasi, Babak
Abstract :
Neurotoxins induce undesirable changes in aquatic organisms. This project assessed the effects of 78.15 g/L aspartame on expression changes of two biomarkers of neurotoxicity (glial fibrillary acidic protein-GFAP and myelin basic protein- MBP) in Cyprinus carpio brain tissue. RNA was extracted from brain tissue. The results of qPCR indicated an increase of 2.5 and 4.2 of MBP and GFAP expression. Elevated levels of these biomarkers suggest the probability of aspartame neurotoxicity. Identifying and monitoring nerve damage using biomarkers can make it easier to diagnose injuries earlier than current methods and provide an opportunity to develop future treatments
Keywords :
Aspartame , Biomarker , Common carp , Neurotoxicity , Gene expression
Journal title :
Journal of The Persian Gulf (Marine Sciences)
