The Effects of Nigella Sativa (Corek Otu) Seeds on Plasma Nitric Oxide, Malondialdehyde and Whole Blood Glutathione Concentrations in Geese

In this study, the effects of Nigella sativa seeds on plasma malondialdehyde (MDA), nitric oxide (NO) and whole blood glutathione (GSH) concentrations were investigated in geese. For this purpose, 8 Anser anser breed geese were fed with a diet containing 5 % ground Nigella sativa seeds for 6 weeks. Blood samples were properly collected from V. subcutenea ulnaris at the beginning and every week of the study for 6 weeks. Biochemical analysis of plasma MDA and blood GSH concentrations were spectrophotometrically measured on a weekly basis according to the methods of Yoshioka et all. and Beutler et all. respectively. Plasma NO concentration was measured by the method of Miranda et all. with a microplate reader. Alterations in measured parameters were determined with respect to the weeks of study. While MDA concentration was significantly decreased at the 2nd week and continued to be decreased compared to 1st week, blood GSH level was significantly increased and remained high compared to 1st week of study. However, no alteration was found in plasma NO concentration among weeks. In conclusion, Nigella sativa administration for 6 weeks resulted in significant decrease in MDA levels and significant increase in GSH levels of geese implying that Nigella sativa could be protective against oxidative stress.