Investigations upon the Likelihood of Transformation and Resistance of Na-Tellurite Originated Plasmide at the Soil Bacteria Isolated from Natural Environment

In this study, the inhibitory effects of Na-tellurit compound at the Alkalophilic Bacillus spp. strains isolated from the soil bacteria were investigated. Bacteria were tested against the increasing concentrations of Na-tellurite at 1-180 μg/ml. The isolates of Na-tellurite at the 1-60 μg/ml concentrations mostly showed resistance, but sensitivity was determined at 70-180 μg/ml. Complete (100 %) sensitivity was observed at 180 μg/ml. For Bacillus spp., the MICs of Na-tellurit were found to be 0.8 μg. Plasmid elimination was made using acridine orange (20 μg/ml) and ethidium bromide (10-5 M) for determination of the isolate resistance originated from plasmid against Na-tellurite. In the isolates of Bacillus spp, there were 60.86 % sensitivity, 39.13 % resistance phenomenon and 21.10 % ratio of resistance originated from plasmid against Na-tellurite. Plasmids of B. cereus T112-Na-TeR, carrying the resistance gene against the antibiotic and Na-tellurite, were transferred by protoplast transformation to B. megaterium DSM 32-Na-TeS strain showing sensitivity against antibiotic and Na-tellurit. A number of 108 transformants from the soil isolates were obtained by protoplast transformation. The seperation of plasmid was made by agarous gel electrophoresis and, finally it was determined that the plasmid had 22,514 bp of molecular weight.