Title of article :
Determination of Coxiella burnetii in Cow Abortions by Polymerase Chain Reaction in Erzurum Province
Author/Authors :
KÜÇÜKKALEM, Ömer Faruk Erzurum Veteriner Kontrol Enstitüsü Müdürlüğü, Türkiye , CENGİZ, Seyda Atatürk Üniversitesi - Veteriner Fakültesi - Mikrobiyoloji Anabilim Dalı, Türkiye , KILIÇ ALTUN, Serap Erzurum Veteriner Kontrol Enstitüsü Müdürlüğü, Türkiye , YILDIRIM, Metin Atatürk Üniversitesi - Veteriner Fakültesi - Mikrobiyoloji Anabilim Dalı, Türkiye
Abstract :
Molecular detection of Coxiella burnetii from cow abortions was aimed in this study. One hundred aborted cow foetuses were investigated. DNA extraction was done from foetal tissues (foetal liver and spleen). Primers C.B.-1 (5 -ACT CAA CGC ACT GGA ACC GC-3 ) and C.B.-2 (5 -TAG CTG AAG CCA ATT CGC C-3 ) were used for determination of Coxiella burnetii. Coxiella burnetii was found to be positive by amplification and imaging in the 6 out of 100 samples. As a result, the presence of Coxiella burnetii should be taken into account for cow abortions in the vicinity of Erzurum.
Keywords :
Abortus , Coxiella burnetii , Cow , PCR.
Journal title :
Atatürk University Journal Of Veterinary Sciences
Journal title :
Atatürk University Journal Of Veterinary Sciences
