Effects of Vitamin E and Selenium Combination on Oxidative Response in Lambs Vaccinated Against Caseous Lymphadenitis

The objective of this study was to investigate the effects of vitamin E and selenium (Se) combination on the oxidative response in lambs vaccinated against caseous lymphadenitis (CLA). For this purpose, 40 healthy female Akkaraman lambs, aged two-month-old, unvaccinated against the CLA were used. Lambs were randomly divided into 4 equal groups. Group I: Control, Group II: Vaccine; Group III: Vitamin E+Se; Group IV: Vaccine + Vitamin E+Se. Blood samples were collected from the lambs on days zero and 21st. In order to determine the oxidative response, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities, as well as malondialdehyde (MDA) and nitric oxide (NO) levels were measured. When the values between days zero and 21st compared: the MDA levels showed no change (P 0.05); the GSH-Px (P 0.05) and NO (P 0.001) decreased in all groups; the SOD activity increased in Group I (P 0.05) as compared to other groups while the CAT activity decreased in Group I, II and IV (P 0.001). When the values within the same day compared; the GSH-Px activity, the MDA and NO levels showed no difference (P 0.05). On day zero, the CAT activity showed significant difference between Group III and Group I, II and IV (P 0.05). On day 21st, the SOD activity in Group I was higher (P 0.05) while the CAT activity was lower (P 0.05) than those in other groups. In conclusion, in Akkaraman lambs vaccinated against caseous lymphadenitis, the administrated dose of vitamin E+Se combination had no marked effect on the SOD, CAT, GSH-Px activities as well as the MDA and NO levels.