Determination of Neospora caninum and Toxoplasma gondii in Sheep and Goat Fetuses Using Dublex PCR, Immunohistochemistry, and Immunflourescence Methods

Bacterial and viral agents have been commonly started to investigate in abortions which are seen in sheep and goats in our country. The study, which involves three different methods (dublex PCR, immunohistochemistry and immunofluorescence) onto sheep and goat abortions which are caused by Neospora caninum and Toxoplasma gondii agents has been never performed before. This study is aimed to determination of Neospora caninum and Toxoplasma gondii in sheep and goat abortions. Neospora caninum was not determined in samples of inspected 16 sheep and 7 goat abortions. However, Toxoplasma gondii was detected into one sheep abortion with dublex PCR, immunohistochemistry and immunofluorescence methods, it was also detected into one goat abortion with dublex PCR, immunohistochemistry methods. The antigens, which was immnunohistochemically positive detected in samples of sheep were observed into the liver, but they were observed into myocard in addition to liver in samples of goat abort. In contrast to any positiveness was not detected in dyeing that was performed with immunofluorescence methods, positiveness was observed into spleen of sheep abortions. Consequently, this project suggested that existence of Toxoplasma gondii has been first revealed by using duplex PCR together with immunohistochemistry and immunofluorescence methods in sheep and goat abortions in our country.