Title of article :
Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate
Author/Authors :
al-abachi, mouayed q. university of baghdad - college of science - department of chemistry, Baghdad, Iraq , al-najjar, nada ali university of baghdad - college of science for women - department of chemistry, Baghdad, Iraq
Abstract :
Flow-injection (FI) spectrophotometric method has been developed for the analysis of thymol in pharmaceutical preparations. The method is based on organic coupling reaction between thymol and 4-amino antipyrine in the presence of alkaline medium to form an intense stable red color complex with copper nitrate that has a maximum absorption at 490 nm. Optimum conditions for determination of the drug was investigated .The calibration graph was linear over the range of 5-500 μg.ml-1 of thymol . The limit of detection (LOD) and limit of quantification (LOQ) were 1.81 μg mL-1 and 3.60 μg mL-1 respectively .The proposed method was applied satisfactorily to the determination of thymol in mouth wash preparations. The procedure is characterized by its simplicity, accuracy and precision.
Keywords :
Thymol , Flow , injection Spectrophotometric determination , 4 , Aminoantipyrine , copper nitrate
Journal title :
Baghdad Science Journal
Journal title :
Baghdad Science Journal
