LUCIGENIN-AND LUMINOL-ENHANCED CHEMILUMINESCENCE IN MECHANICAL INJURED ROOTS OF CORN ( Zea mays L. )

The chemiluminescence ( CL ) of roots from com ( Zea mays L. ) seedling , after mechanical injury , has been observed by lucigenin - or luminal dependent chemiluminescence ( LCDC ) and ( LMDC ) respectively . The light emission in injured roots system was blocked by NaCN in LMDC system, whereas ascorbic acid addition resulted about 50% and 70% reduction in light emission in LMDC ~and LCDC systems respectively. In another experiment of injured roots extracts , the Light emission was increased by the addition of H20 2 in LCDC and LMDC systems , whereas NaCN addition has blocked effect in ( LMDC ) system. The suggested results in this study shows the effect of CL substrates , such as luminal or lucigenin and the addition compounds ( NaCN or ascorbic acid radical scavenger ) on the CL intensity , may be proved the participation of reactive oxygen species, mainly superoxide anion , and the free radical mechanism in the CL- injured roots reaction of com plant seedlings