Title of article :
Purification and characterization of camel liver L-asparaginase
Author/Authors :
Maharem, Tahany M. Ain Shams University - Faculty of Science - Department of Biochemistry, Egypt , Sabry, Gilane M. Ain Shams University - Faculty of Science - Department of Biochemistry, Egypt , Mohamed, Mohamed R. Ain Shams University - Faculty of Science - Department of Biochemistry, Egypt , Emam, Manal A. Ain Shams University - Faculty of Science - Department of Biochemistry, Egypt
Abstract :
L-asparaginase from camel liver was isolated and purified by heat denaturation followed by QAE-Sephadex A-50 column chromatography and SP-Sepharose column chromatography. The purified camel liver L-asparaginase had a molecular weight of 180 kDa (consistent with a homotetramer) and a pI value of 8.6. The enzyme was thermostable with relative structure rigidity and an optimum temperature at 65°C . It had a pH optimum at 9.6 and was stable for storage at 4°C in the refrigerator for 7 days
Keywords :
Camel , liver , purification , L , asparaginase
Journal title :
The Egyptian Journal of Biochemistry and Molecular Biology
Journal title :
The Egyptian Journal of Biochemistry and Molecular Biology
