Author/Authors :
Nna, Emmanuel University of Nigeria - Faculty of Health Sciences and Technology - Safety Molecular Pathology Laboratory, Nigeria , Madukwe, Jonathan National Hospital - Department of Histopathology, Nigeria , Egbujo, Ejike University of Jos Teaching Hospital - Meena Histopathology and Cytology Laboratory, Nigeria , Obiorah, Chris University of Port Harcourt Teaching Hospital - Department of Histopathology, Nigeria , Okolie, Charles University of Jos Teaching Hospital - Department of Histopathology, Nigeria , Echejoh, Godwin University of Jos Teaching Hospital - Department of Histopathology, Nigeria , Yahaya, Amina University of Jos Teaching Hospital - Department of Histopathology, Nigeria , Adisa, James University of Jos Teaching Hospital - Department of Histopathology, Nigeria , Uzoma, Ijeoma University of Nigeria - Faculty of Health Sciences and Technology - Safety Molecular Pathology Laboratory, Nigeria
Abstract :
Objective: To measure the transcript levels of Aurora kinases and compare them to their immunoreactivity patterns in prostate tumors. Materials and Methods: A total of 26 cases of prostate cancer (PCa) and 38 cases of benign nodular hyperplasia (BPH) were sampled from archived formalin-fixed paraffin-embedded tissues. Tissue sections were lysed, total RNA extracted and cDNA made by random hexamer priming while slide sections were immunostained for the kinases. Normalized relative quantitation was performed for all the kinases using real-time PCR on TaqMan chemistry. Results: The immunoreactivity profile showed 15.4, 53.8 and 30.7% positivity for Aurora kinases A, B and C in PCa cases, respectively, while the positivity was 76.3, 73.7 and 84.2% for the same kinases in BPH cases. The immunoreactivity was preponderant on epithelial tissue compared to stromal component. Conclusion: Aurora kinases were significantly overexpressed in BPH cases compared to PCa cases. At the transcript level, there was no significant differential expression in the kinases between PCa and BPH cases.
Keywords :
Aurora kinases , Aurora kinase A , Aurora kinase B , Aurora kinase C , Gene expression , Prostate cancer
