Synthesis of Novel Series of 1-(6-Hydroxy-4-(1H-indol-3-yl)-3,6-dimethyl- 4,5,6,7-tetrahydro-1H-indazol-5-yl)ethan-1-oneas Evaluations of their Antimicrobial Activity with Insilco Docking Study

This research study discusses the silico design, synthesis, and biological evaluation of novel effective phenyl, indole, 3,4-dimethyl substituted 4,5,6,7-tetrahydro-1H-indazole derivatives. The novel multi- substituted indazole derivatives (5A-5J) was synthesized from the treatment of hydrazine hydrates in MeOH/H+ with multi substituted clohexanone derivatives (4a-4j). The final scaffold was characterized with the help of spectroscopic data such asIR, 1H NMR, 13C NMR, and mass spectra. The compound 5A, 5D, and 5F shows excellent antibacterial activity and the compounds 5B, 5C, 5H 5I and 5J exhibited moderate antibacterial activity against the S. aureus, Bacillus subtilis and E.Coli. Finally, the molecular docking studies showes that the compound 5D and 5F scaffolds display excellent bonding mode of interactions with the active site of DNA gyrase 1KZN enzyme.