Bcl2-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells

There has been a prevailing trend in the application of herbal medicine as cancer therapeutics. Calotropis procera is an ayurvedic plant applied to ameliorate various illnesses. There is no report on the antitumor effects of the root of the plant on canine tumors, although it has been used for the treatment of various diseases in human medicine. The objective of the present study was to investigate the antitumor potential of ethanolic root extract of C. procera against canine mammary tumor cell line (CF41-Mg). MTT, western blot, and flow cytometry assays were carried out to evaluate the possible cytotoxicity and apoptosis induction of the extract. MTT results showed that the extract had a potent cytotoxic activity in a dosedependent manner with an IC50 of 9.00 μg mL^-1. Based on the results of flow cytometry and western blotting, IC50 concentration of the extract induced significant apoptosis in the studied cell line, possibly through downregulation of Bcl-2 expression. The results of the present study clearly indicated that the root extract of C. procera had promising anticancer activity and could be considered as a candidate for the treatment of mammary tumors.