Title of article :
Molecular identification of Fusarium spp. isolated from tomato plant in Iraq and China
Author/Authors :
fayyadh, mohammed a. university of basrah - college of agriculture - department of plant protection, Basrah, Iraq , al-badran, adnan i. university of basrah - college of science - department of biology, Basrah, Iraq , al-jaafari, iman s. university of basrah - college of agriculture - department of plant protection, Basrah, Iraq
Abstract :
This study was conducted to identify Fusarium spp. isolated from tomatoplant in Iraq and China. A total of 12 isolates from Iraq (1-12) and four isolates fromChina (M1-M4) were used in this study. Based on Morphological characteristics (color,growth pattern, Macro and Micro-conidia shape) high differences between Fusariumisolates were found. Sensitivity test to the fungicide carbendazim revealed that one thirdof isolates(4/12) from Iraq had EC50 values over than 1000μg/ml indicated that manyIraqi isolates have developed resistance to carbendazim. Based on ITS sequences,Fusarium isolates were identified as follow, isolates 1, 3, 5, 6, 7, 10 were identified asFusarium oxysporum, isolates 8, 11 as F. solani, isolates 12, M1 and M3 as F.moniliforme, isolates 2, M2 and M4 as F. proliferatum, F. chlamydosporum and F.kyushuense , respectively.
Keywords :
RAPD , PCR , Fusarium spp , tomato plant , Iraq , China
Journal title :
Basrah Journal Of Agricultural Sciences
Journal title :
Basrah Journal Of Agricultural Sciences
