The Effect of SNP c.100800G > A on CAST|Cfr13I Gene Polymorphisms with Ultrasound Imaging of Meat Characteristics and Growth Traits in Bali Cattle

Bali cattle are known as native cattle from Indonesia, which commonly utilized as beef-producing animals. Calpastatin gene (CAST) plays essential role in meat quality. The aim of this study was to verify the effect of single-nucleotide polymorphism (SNP) c.100800G>A on the CAST|Cfr13I gene associated with meat characteristics and growth traits in Bali cattle. The meat characteristics, growth traits profile, and blood samples of Bali cattle (n=52 animals) obtained from BPTU Bali Cattle Denpasar, Bali Province. Compari-son used were Belgian Blue (n=30 animals), Wagyu (n=7 animals), Limousin (n=14 animals), and Perana-kan Onggole (PO) (n=30 animals). Ultrasound measurement was conducted to study the meat characteris-tics. The examination of the CAST gene polymorphisms used polymerase chain reaction-restriction frag-ment length polymorphism (PCR-RFLP) method digested by Cfr13I enzyme and the analysis of effect of the CAST|Cfr131 gene on meat characteristics and growth traits in Bali cattle used t test with SAS 9.4 pro-gram. Bali cattle revealed polymorphic homozygous genotype (GG) and heterozygous genotype (GA) with allele frequencies of G and A were 0.923 and 0.077, respectively. In comparison, other breeds beef cattle, including Belgian Blue, Limousin, Wagyu, and PO, showed only the GG genotype with allele frequency of G was 1.000. The CAST|Cfr13I gene showed no significant association (P>0.05) with Bali cattle meat char-acteristics and growth traits. In conclusion, the SNP c.100800G > A may not be purposed as a marker for Bali cattle meat characteristics and growth traits. It was suggested that further study with a higher number of animals would be necessary to validate the effect of the SNP c.100800G > A on the CAST gene with the actual beef cutting of Bali cattle.