Author/Authors :
Abdolahi, Mahnaz Pediatric Pathology Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rasouli, Shima Department of Immunology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Babaie, Delara Department of Allergy and Clinical Immunology - Mofid Children’s Hospital - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Dara, Naghi Pediatric Gastroenterology - Hepatology and Nutrition Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Imanzadeh, Farid Pediatric Gastroenterology - Hepatology and Nutrition Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Sayyari, Aliakbar Pediatric Gastroenterology - Hepatology and Nutrition Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rouhani, Pejman Pediatric Gastroenterology - Hepatology and Nutrition Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Khatami, Katayoun Pediatric Gastroenterology - Hepatology and Nutrition Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Kazemiaghdam, Maryam Pediatric Pathology Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Nilipour, Yalda Pediatric Pathology Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Khoddami, Maliheh Pediatric Pathology Research Center - Research Institute for Children’s Health - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ghadimi, Farah Department of Allergy and Clinical Immunology - Mofid Children’s Hospital - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mousavinasab, Fatemeh Department of Immunology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mesdaghi, Mehrnaz Department of Immunology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Aim: Considering the allergic basis of Eosinophilic esophagitis (EoE), this study was conducted to evaluate peripheral blood Tregs in children with EoE.
Background: Eosinophilic esophagitis (EoE) is an allergic inflammatory disease of gastrointestinal tract. Regulatory T cells (Tregs) have a confirmed role in allergic disorders.
Methods: Children with EoE, gastroesophageal reflux disease (GERD) and healthy controls (HC) (10 subjects in each group) were recruited after diagnosis by a pediatric gastroenterologist and allergist. After obtaining informed written consent, peripheral blood was obtained. Peripheral blood mononuclear cells were isolated by Ficoll gradient centrifugation. Flowcytometry was used to enumerate peripheral blood Tregs (CD4+CD25+FOXP3+ gated lymphocytes were considered as Tregs).
Results: CD4+ gated lymphocytes significantly increased in EoE and GERD groups compared to HC group (p= 0.018). Tregs also was significantly increased in EoE in comparison to HC group (p=0.016). There were no statistically significant differences in Tregs of EoE as compared to GERD subjects (p=0.085).
Conclusion: Peripheral blood Tregs increase in patients with EoE as compared to healthy controls, which may be indicative of a feedback mechanism to regulate inflammatory responses.
