Author/Authors :
Bahramabadi , Reza Pathology and Stem Cell Research Center - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Honarvar, Zahra Department of Obstetrics and Gynecology - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Iranpour, Maryam Pathology and Stem Cell Research Center - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Kazemi Arababadi, Mohammad Immunology of Infectious Diseases Research Center - Research Institute of Basic Medical Sciences, and Department of Laboratory Sciences - Faculty of Paramedicine - Rafsanjan University of Medical Sciences, Rafsanjan, Iran , Dehesh, Tania Department of Biostatistics and Epidemiology - Faculty of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Dabiri, Bahram Pathology and Stem Cell Research Center - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Mortezaeizadeh Anari, Abbas Pathology and Stem Cell Research Center - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Amirpour Rostami, Sahar Pharmaceutics Research Centre - Institute of Neuropharmacology - Kerman University of Medical Sciences, Kerman, Iran , Salajegheh, Mohammad Pathology and Stem Cell Research Center - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Dabiri, Shahriar Pathology and Stem Cell Research Center - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran
Abstract :
Background: Uterine cervical malignancy is one of the commonly detected malignancies related to the human papillomavirus (HPV) and is increasing incidentally in developing countries. Therefore, the use of an efficient diagnostic method is required as an effectual step for cervical cancer prevention and treatment. The purpose of the study was to diagnose various types of HPV in the cervical cytology specimens in the South-East of Iran.
Methods: This cross-sectional study was performed on 1079 cervical fluid cytology specimens referred for two years, between 2018-2020. Polymerase chain reaction (PCR) and hybridization (INNO-LiPA HPV Genotyping EXTRA II assay) were used to determine HPV DNA and their genotypes, respectively.
Results: HPV was positive in 37.7% (407 of 1079) patients with a mean age of 34.62 ± 8.82. Among positive cases, 252 (62%) had only one HPV genotype and 155 (38.05%) had multiplex HPV genotypes, which included 94 (60.7%), 38 (24.6%), 18 (11.6%) and 5 (3.2%) cases with two, three, four and five or more genotypes, respectively. The samples with multiple strains revealed 31 HPV genotypes with the four most prevalent being HPV6 (14.7%), HPV16 (10.9%), HPV53 (9.6%) and HPV51 (5.9%).
Conclusion: HPV infection is the main health challenge for women that requires improved health service programs and appropriate epidemic vaccination.
