Author/Authors :
Abdella, Sintayehu Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Waktole, Hika Addis Ababa University Colleges of Veterinary Medicine and Agriculture (AUCVMA) - Debrezeit, Ethiopia , Mamo, Gezahegn Addis Ababa University Colleges of Veterinary Medicine and Agriculture (AUCVMA) - Debrezeit, Ethiopia , Ali, Abraham Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Getahun, Desalegn Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Mulugeta, Yimer Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Ashenafi, Bayeh Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Getahun, Garoma Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , File, Ebsa Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Tadesse, Yirgalem Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Alemu, Anbereber Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Birhanu, Endalkachew Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Girma, Endalkachew Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Ferede, Henok Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Getachew, Abebe Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia , Aklilu, Mesfin Ethiopian Public Health Institute (EPHI), Addis Ababa, Ethiopia
Abstract :
Direct rapid immuno-histochemical test (DRIT) is used as a gold standard method for rabies virus detection. The present study aimed to compare and evaluate DRIT with direct fluorescent antibody test (DFAT) to use equivalently as one of rabies diagnosing methods in areas where DFAT is not accessible. The method is based on the capture of rabies nucleoprotein (N) antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counterstaining with hematoxylin. The test was performed in parallel with the standard DFAT and mice inoculation test (MIT) using 100 brain specimens from various species of animals. The majority of them were dogs (n =88), followed by cats (n =8), cattle (n =3), and donkey (n =1), and also from those samples that were tested by DRIT and DFAT, we randomly selected and tested 12 brain samples by MIT. The results indicated that 63% of the tests were positive by DFAT and 64% were positive by DRIT. A slight difference was observed in such a way that one sample was negative by DFAT but positive by DRIT and MIT. Although, further laboratory and field examinations are essential, our findings were providing and remark the potential value of the DRIT for countries with limited diagnostic resources.
