Author/Authors :
Mirmoeini, Tayebehalsadat Department of Biology - Islamshahr Branch - Islamic Azad University, Islamshahr, Iran , Pishkar, Leila Department of Biology - Islamshahr Branch - Islamic Azad University, Islamshahr, Iran , Kahrizi, Daniel Department of Agronomy and Plant Breeding - Razi University, Kermanshah, Iran , barzin, Giti Department of Biology - Islamshahr Branch - Islamic Azad University, Islamshahr, Iran , Karimi, Naser Plant Physiology Laboratory - Department of Biology - Faculty of Science - Razi University, Kermanshah, Iran
Abstract :
Camelina is an oilseed and re-emerging plant that requires a lot of research on its oil production process. This study was conducted to investigate the effect of biosynthesized silver nanoparticles on the expression level of FAE1 and FAD2 in Soheil cultivar of Camelina oilseed plant based on a completely randomized design with four replications in 2018-2019. The aqueous extract of Camelina leaf and silver nitrate salt was used to prepare nanoparticles. Experimental treatments included 0.5, 1, 2, and 3 mg /L silver nanoparticles. After preparing foliar samples for all treatments, RNA extraction, cDNA synthesis, and temperature gradient determination, the Real Time PCR reaction was used to study gene expression patterns. The data were then analyzed using GenEX and SAS software. Results showed that the effects of silver nanoparticles on FAE1 and FAD2 gene expression were significant (p<0.05), showing an increase with increasing silver nanoparticle concentration. The highest enhancement was observed at 3 mg/L silver nanoparticles.
Keywords :
biosynthesize , Camelina , gene expression , nanoparticles , Real Time PCR
