Author/Authors :
Salemi-Najaf abadi, S Department of Microbiology - Falavarjan Branch - Islamic Azad University - Isfahan, Iran , Doudi, M Department of Microbiology - Falavarjan Branch - Islamic Azad University - Isfahan, Iran , Tahmourespour, A Department of Basic Medical Sciences Isfahan - (khorasgan)Branch - Islamic Azad University - Isfahan, Iran , Amiri, Gh. R Department of Basic Science - Falavarjan Branch - Islamic Azad University - Isfahan, Iran , Rezayatmand, Z Department of Plant Biology - Falavarjan Branch - Islamic Azad University - Isfahan, Iran
Abstract :
Nanotechnology is the science to create products with enormous potential to treat diseases. Diabetic ulcers and their infections have affected many people around the world, and many people die each year from infections caused by microorganisms in these ulcers. The aim of this study was to investigate the antibacterial properties of chitosan nanoparticles against Escherichia coli isolated from diabetic wounds. Morphological evaluation and substructure of nanoparticles synthesized by ion gelling method were performed by XRD and TEM. Investigation of the antibacterial properties of chitosan nanoparticles on Esherichia coli by qualitative agar well diffusion test and microdilution was performed to the concentration of 0.064 g/l to 0.256 g/l. The size of chitosan nanoparticles in this study was 100 nm and spherical shape. The sizes of the inhibition zone were different according to the type of bacteria and the concentrations of chitosan, the maximum zone diameter 20 mm in concentration 0.256 g.l being observed.
Keywords :
Nanotechnology , Escherichia coli , Diabetic ulcers , Chitosan nanoparticles , Antibacterial effect
