Author/Authors :
Taraghian, Mina Department of Microbiology - School of Basic Sciences - Islamic Azad University Hamedan Branch, Hamedan , Hanif, Helena Department of Biology - Faculty of Education - Herat University, Herat, Afghanistan , Mousavi, Parisa Skin Diseases and Leishmaniasis Research Center - Isfahan University of Medical Sciences, Isfahan , Baharlooeyan Cheshmeh, Zahra Medical Laboratory Department - Laboratory Expert - University of Medical Sciences, Hamedan , Samei, Azam Department of Laboratory Sciences - School of Allied Medical Sciences - Kashan University of Medical Sciences, Kashan , Abdollahi, Abbas Department of Microbiology - Fasa University of Medical Sciences, Fasa , Vazini, Hossein Nursing Department - Basic Sciences Faculty - Islamic Azad University Hamedan Branch, Hamedan
Abstract :
Background: Leishmaniasis is one of the main vectors borne and neglected tropical
parasitic diseases. T cell cytokine responses are highly important in the presentations
of disease such as control or progression, and understanding of the host
immunological response is valuable in diagnosis, follow-up, and vaccine designs.
In the current study, the profile of IFN-ɤ, TNF-α, and IL-10 cytokines was investigated
through the ELISA technique in PBMCs isolated from antimony resistance
and susceptible patients.
Methods: In this experimental study, 54 patients with healing (n=27) or nonhealing
(n=27) CL were recruited. Lesion samples were collected to determine the
genotype of Leishmania spp. and peripheral blood mononuclear cells (PBMCs)
were obtained to evaluate the cytokines profiles using soluble Leishmania antigen
(SLA) and phytohaemagglutinin (PHA) mitogen. Cytokines were assessed by the
ELISA technique
Results: The IFN-ɤ and TNF-α cytokines were significantly increased in the healing
group treated with both SLA antigen and PHA mitogen (P<0.001). The level
of IL-10 was significantly increased in non-healing and significantly declined in
healing groups (P<0.001).
Conclusion: The profile of IFN-ɤ, TNF-α, and IL-10 cytokines are crucially associated
with the response of treatment.
Keywords :
Cutaneous leishmaniasis , IFN-gamma , TNF-alpha , Iinterlokin-10 , Immunological response
