Author/Authors :
Ghanbari Johkool, Morteza Metabolic Diseases Research Center - Research Institute for Prevention of Non-Communicable Diseases - Qazvin University of Medical Sciences, Qazvin , Vafae Eslahi, Aida Department of Parasitology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran , Badri, Milad Metabolic Diseases Research Center - Research Institute for Prevention of Non-Communicable Diseases - Qazvin University of Medical Sciences, Qazvin , Hooshmand, Elham Department of Parasitology - Faculty of Veterinary Medicine - Islamic Azad University Rasht Branch, Guilan , Pirestani, Majid Department of Parasitology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran , Jablonski, Daniel Department of Zoology - Comenius University in Bratislava, Mlynská dolina, Slovakia , Jafari,Rasool Department of Medical Parasitology and Mycology - Faculty of Medicine - Urmia University of Medical Sciences, Urmia , Dalimi, Abdolhossein Metabolic Diseases Research Center - Research Institute for Prevention of Non-Communicable Diseases - Qazvin University of Medical Sciences, Qazvin
Abstract :
Background: The genus Abbreviata (Spirurida: Physalopteridea) currently contains
47 species. Physalopteridae nematodes infect a large number of vertebrates, including
mammals, birds, reptiles and amphibians. The current study is a report of
the first morphological and molecular identification of A. kazakhstanica (Spirurida:
Physalopteridea) in Pseudopus apodus in Iran.
Methods: Eleven road-killed P. apodus, were collected from, Iran during 2016-
2018. The nematodes were isolated from stomach. After morphological study, the
genomic DNA of the parasites was extracted using CTAB method. The DNA was
used for PCR amplification of cytochrome c oxidase subunit I (cox1). The PCR
products were sequenced, the sequence data were analyzed and multiple alignments
were conducted using the Clustal Omega.
Results: After detailed microscopic examination, the A. kazakhstanica was identified.
The cox1 sequences confirmed the species of helminth. The new sequences
of A. kazakhstanica were submitted to GenBank under the accession number
MK578751-2.
Conclusion: Regarding the limited data on parasitological status of Iranian reptiles,
more specific and comprehensive investigations are needed to identify the
parasitic fauna.
Keywords :
Abbreviata kazakhstanica , Molecular identification , Pseudopus apodus , Physalopteridea , Iran
