Author/Authors :
Dhanasegaran, K. Department of Biomedical Science - Faculty of Science - Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia , Djearamanea, S. Department of Biomedical Science - Faculty of Science - Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia , Lianga, S.X.T. Department of Biomedical Science - Faculty of Science - Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia , Wong, L.S. Life Science Division - Faculty of Health and Life Sciences - INTI International University, Nilai, Negeri Sembilan, Malaysia , Kasivelu, G. Centre for Ocean Research (DST-FIST Sponsored Centre) - Sathyabama Institute of Science and Technology, Chennai, India , Lee, P.F. Department of Mechatronics & Biomedical Engineering - Lee Kong Chian Faculty of Engineering and Sciences - Universiti Tunku Abdul Rahman, Bandar Sungai Long, Malaysia , Lime, Y.M. Department of Pre-Clinical Sciences - Faculty of Medicine and Health Sciences - Universiti Tunku Abdul Rahman, Bandar Sungai Long, Malaysia
Abstract :
The application of nanotechnology has brought about advancements in environmental and medical applications. Recently, zinc oxide nanoparticle (ZnO NP) is commonly used to treat a wide range of bacterial and fungal skin infections due to its antimicrobial property. This investigation was intended to study the antimicrobial eect of ZnO NP on Pseudomonas aeruginosa by testing the bacterial inhibition and the morphological damages caused by ZnO NP to P. aeruginosa. The results of this study exhibited a typical dose-dependent and signifcant (p < 0:05) inhibition of the growth of P. aeruginosa treated with 5 to 150 gg/mL of ZnO NP. The polysaccharides and polypeptides from P. aeruginosa cell wall were found to be associated with the attachment of ZnO NPs to bacterial cells as illustrated in the Fourier Transform Infrared (FTIR) spectrum. Furthermore, Scanning Electron Microscopy (SEM) images displayed the surface attachment of ZnO NPs on bacteria and the morphological changes, such as disrupted cell wall integrity, cell bending
Keywords :
Zinc oxide nanoparticles , Pseudomonas aeruginosa , Bacterial growth inhibition , Antibacterial property , Scanning electron microscopy , Morphological damage
