evaluating osteocalcin and osteonectin in serum male patients with type 2 diabetes mellitus and periodontitis

biomarkers play an essential role since they are good predictor factors for several diseases. accordingly, we attempted to assess the level of osteocalcin and osteonectin in patients suffering from periodontitis in type 2 diabetes mellitus compared to healthy control. 120 male persons were included in the current study collected from the albaladyat specialist center for dentistry in iraq. patients have been divided into three groups (30 patients with type2 diabetic mellitus, 30 patients with periodontitis, 30 patients with diabetic mellitus with periodontitis), and 30 healthy subjects in a control group. clinical periodontal parameters were determined for all the studied groups. additionally, serum was collected from each subject to determine osteocalcin and osteonectin levels by the elisa method.  the results revealed a significant difference between study groups and controls regarding the levels of osteonectin and osteonectin at the pvalue (0.003 and 0.0001). moreover, the current study results showed a direct association between each of osteonectin, osteocalcin with clinical periodontitis parameters. moreover, analysis by the roc curve showed that both osteonectin and osteocalcin might represent the strongest markers for diagnosis of periodontitis in diabetic patients. levels of the studied biomarkers suggest suitable biomarkers for early detection of the sixth diabetic complication (periodontitis).