protective effects of the combination of the herbal compound against inflammation related to obesity and colitis induced by diet in mice

objective: high-fat diet (hfd) rises the susceptibility of both obesity and consequently inflammatory bowel disease (ibd). we designed a study to investigate the improving effects of herbal extract (he, the combination of turmeric, ginger, boswellia, and cat’s claw extract) on the risk of high ages-fat diet 60% (hfd) mice induced colitis and obesity. materials and methods: four-week-old c57bl/6 male mice after 2 weeks adaptation with normal diet were fed with either hfd or normal diets. after 6 weeks of being on diet, animals received he for 16 weeks. obesity index markers were determined as well as histological studies using h e (hematoxylin-eosin) staining. colonic expression of il-1β was determined. data analysis was performed by utilizing kruskal-wallis and mann-whitney test for post-hoc comparisons, and spss (version 17.0) and graphpad prism software (version 8.0, usa). results: he decreased histological scores (by 6-fold) in hfd diet-fed mice, and reduced myeloperoxidase activity (by 2.2-fold), and ratio of colon weight to length (by 4-fold) in hfd diet-fed mice. moreover, he prevented intestinal permeability through the restoration of zo-1 (by 4-fold) and immune homeostasis by modulation of il-1β (by 2.4-fold) expression. conclusion: hfd induced obesity-associated colitis. he decreased the colitis symptoms in hfd diet-fed mice, with the reduction of inflammation.